Role of microtubules in the intracellular transport of growth hormone |
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| Authors: | Dr S L Howell Margaret Tyhurst |
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| Institution: | (1) School of Biological Sciences, University of Sussex, Falmer, Brighton, Sussex, UK, Great Britain;(2) Department of Biochemistry, Charing Cross Hospital Medical School, Fulham Palace Road, W6 8RF London, Great Britain |
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| Abstract: | Summary Pulse-chase experiments utilising(3H)leucine have been used to study the effects of colchicine and vinblastine on intracellular transport and secretion of newly synthesised growth hormone from rat anterior pituitary fragments. Growth hormone was isolated from medium and fragments by polyacrylamide gel electrophoresis. When colchicine or vinblastine, which disrupt microtubules, were added immediately after pulse labelling, inhibition of the subsequent secretion of newly synthesised growth hormone was detected throughout the succeeding 5 h. Similar inhibition was seen if the drugs were added after a 1 h delay. However, if colchicine or vinblastine were added only after a 2 h chase incubation, then no significant effect on subsequent release of labelled growth hormone was seen. The results suggest that these agents may inhibit the transport of newly formed growth hormone storage granules from the Golgi complex to the cytoplasmic pool. Microtubules do not appear to be involved in the mechanism of the final secretion of newly synthesised hormone by exocytosis.These studies were supported by grants from the Medical Research Council and British Diabetic Association |
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| Keywords: | Growth hormone Somatotrophs Microtubules Colchicine vinblastine |
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