Fas 相关死亡结构域蛋白在乳腺癌中表达的临床病理研究

目的:探讨Fas相关死亡结构域蛋白(Fas-associated death domain protein,FADD)在乳腺癌中表达的临床病理学意义。方法:收集乳腺癌病例及相应的临床资料包括随访资料,应用免疫组织化学技术检测乳腺良性病变,有/无淋巴结转移的乳腺癌及配对淋巴结转移灶中FADD的表达,观察分析FADD表达与乳腺癌患者年龄、肿块大小、临床分期、组织学类型和分级、雌孕激素受体水平等临床病理指标间的关系。结果:免疫组化检测结果显示良性乳腺病变组中FADD的阳性表达率(85.1%,40/47)与无淋巴结转移的乳腺癌组(45.8%,38/83),伴有淋巴结转移的乳腺癌组(67.3%72/107)和淋巴结转移灶(45.8%,49/107)组织中FADD阳性表率均有显著性差异(P值分别0.001,=0.022和0.001);此外,伴有淋巴结转移的乳腺癌组中FADD阳性表达率也均与其它三组中FADD阳性表达率之间具有显著性差异(P值分别为0.003,0.001和0.022)。FADD与患者的确诊年龄(P=0.049)和淋巴结转移有显著性相关(P=0.003),与肿瘤大小、临床分期、组织学类型、组织学分级、雌孕激素受体及cerb B-2的表达情况和月经史无明显相关性(P0.05)。生存分析显示FADD阳性表达的患者较FADD阴性患者的生存期更短。结论:FADD与乳腺癌淋巴结转移和预后有关。

Clinicopathological Study on the Expression of Fas-associated Death Domain Protein in Breast Cancer

Objective:To investigate the clinicopathological significance of Fas-associated death domain protein (FADD) expression in breast cancer.Methods:The clinical data of breast cancer cases and relevant clinical data were collected. The expression of FADD in breast benign lesion, breast cancer with or without lymph node metastasis and paired lymph node metastasis was detected by immunohistochemical technique. The relationship between FADD expression and clinical pathological parameters of breast cancer patients, such as age, tumor size, clinical stage, histological type and grade, estrogen and progesterone receptor level, and so on was analyzed.Results:Immunohistochemistry results showed FADD positive expression rate of benign breast lesion group (85.1%, 40/47), breast cancer group without lymph node metastasis (45.8%, 38/83), breast cancer group with lymph node metastasis(67.3%,72/107) and paired lymph metastasis foci (45.8%, 49/107). There are significant difference between breast cancer without metastasis, breast cancer with metastasis, lymph node foci and benign breast lesion group (respectively P < 0.001, = 0.022, < 0.001); in addition, FADD positive expression rate of breast cancer grou with lymph node metastasis was higher than the other three groups (P = 0.003, < 0.001 and 0.022, respectively). FADD expression is significantly associated with the patient''s age at diagnosis (P = 0.049) and lymph node metastasis (P = 0.003), and there is significant correlation to tumor size, clinical staging, histological type, histological grade, estrogen and progesterone receptors and c-erbB-2 expression situation and menstrual history (P > 0.05). Survival analysis showed that patients with FADD positive expression had shorter survival time than FADD negative patients.Conclusion:FADD is associated with lymph node metastasis and prognosis of breast cancer.