miR-155 通过p53/p21 调控前列腺癌细胞周期

目的:探讨miR-155对前列腺癌细胞周期的影响及其分子机制。方法:通过转染anti-miR-155抑制前列腺癌DU145和PC-3细胞中miR-155水平后,采用流式细胞术观察细胞周期的变化,western blot和RT-PCR观察p53和p21蛋白及CDK2和cyclin蛋白和m RNA表达的变化。结果:与对照组相比,DU145和PC-3细胞转染anti-miR-155后,G2/M期细胞阻滞,S期细胞数比例显著增加(P0.05),p53和p21蛋白和m RNA表达水平显著增加(P0.01),CDK2和cyclin E蛋白和m RNA表达均显著降低(P0.01)。结论:miR-155可影响人前列腺癌细胞的周期,可能与其调节p53、p21及其下游的CDK2和cyclin E的表达相关。

Effect of miR-155 on the Cell Cycle of Prostate Cancer by p53/p21 Pathways

Objective:To study the effects of miR-155 on the cell cycle of prostate cancer and the underlying mechanisms.Methods:The cell cycles of DU145 and PC-3 cells were evaluated after anti-miR-155 transfection by FACS. p53 and p21 expression were evaluated by western blot and RT-PCR.Results:The cell cycle of DU145 and PC-3 cells were down regulated obviously after anti-miR-155 transfection(P<0.05), and the protein and mRNA expression of p53 and p21 were obviously increased (P<0.01). In addition, the protein and mRNA expression of CDK2 and cyclin E were also decreased obviously (P<0.01).Conclusion:Inhibition of miR-155 could modulate the cell cycles of DU145 and PC-3 cells in vitro by p53/p21 pathways and by down regulating the expression of CDK2 and cyclin E.