hMSH6 和P53在散发性结肠癌中的表达及意义

目的:研究人类mut-s同系物6(hMSH6)和P53蛋白在散发性结肠癌(SCC)中的表达及与SCC病理特征的关系。方法:采用免疫组化Max-Vision二步法检测48例SCC癌组织、距癌灶3~9 cm的癌旁组织及≥10 cm的结肠组织以及45例正常结肠组织中hMSH6、P53的表达情况。结果:癌组织、距癌灶3~9 cm的癌旁组织中,hMSH6的阳性表达率低于距癌灶≥10 cm结肠组织及正常结肠组织,P53的阳性表达率高于距癌灶≥10 cm结肠组织及正常结肠组织,差异比较有统计学意义(P0.05);hMSH6阳性表达与癌灶部位及浸润深度有关(P0.05);P53阳性表达与组织学分级有关(P0.05);hMSH6与P53阳性表达呈负相关(r=-0.403,P0.05)。结论:hMSH6、P53的异常表达参与了SCC发生及发展的过程,hMSH6、P53可作为评价SCC生物学行为的指标。

Expression and Significance of hMSH6 and P53 in Sporadic Colorectal Carcinoma*

Objective:To study expressions of hMSH6 and P53 in sporadic colorectal carcinoma (SCC) and their relation with SCC pathological features.Methods:Immunohistochemical Max-Vision two-step method was applied to detect the hMSH6 and P53 expression in 48 cases of SCC tumor tissue, adjacent tissue of 3~9 cm next to neoplastic foci and colon tissue of 10cm next to neoplastic foci, as well as that in 45 cases of normal colon tissue.Results:The positive expression rate of hMSH6 was lower in cancer tissues and adjacent tissues of 3~9 cm next to neoplastic foci than in colon tissues of 10 cm next to neoplastic foci and normal colon tissues, while the positive expression rate of P53 was higher cancer tissues and adjacent tissues of 3~9cm next to neoplastic foci than in colon tissues of 10 cm next to neoplastic foci and normal colon tissues. The differences were statistically significant (P<0.05). hMSH6 expression was correlated with the foci location and depth of invasion (P<0.05). P53 positive expression was correlated with histological grade (P<0.05). The hMSH6 expression was negatively correlated with P53 (r=-0.403, P<0.05).Conclusion:The abnormal expression of hMSH6 and P53 involved in the process of occurrence and development of SCC. The hMSH6 and P53 can be used as evaluation indicators of SCC biological behavior.