高果糖高脂饮食对小鼠体内能量代谢的影响

目的:高热量物质的过度摄入是导致机体代谢紊乱,诱发2型糖尿病等代谢性疾病的主要原因,本文通过比较高果糖、高脂及高果糖高脂混合喂饲对小鼠体内能量代谢的影响,探索饮食诱发代谢紊乱性疾病的可能发病机制。方法:采用20%高果糖水,60%高脂饲料,及二者混合方式饲养C57BL/6小鼠3个月后,观察各组小鼠24小时内氧气消耗量,二氧化碳生成量,呼吸商及能量消耗的改变。结果:不同饮食喂饲3个月,与对照组小鼠相比,高果糖组、高脂组、及高果糖高脂组小鼠均表现出明显的肝内脂质蓄积,氧气消耗量增加,呼吸商下降,能量消耗增加。结论:过剩的高热量物质摄入导致机体内物质代谢、能量代谢发生改变,糖代谢受损,脂代谢增强,能量代谢方式从糖氧化为主转变为脂氧化供能。

Effects of High Fructose/High Fat Diets on Energy Metabolismin C57BL/6 Mice

Objective:Excess energy intake is a main causal reason for metabolic disorders. To investigate the possible pathogenesis mechanism of metabolic dysfunction, the effects of different high energy foods on energy metabolism were compared.Methods:Oxygen consumption, carbon dioxide production, respiratory quotient and energy expenditure within 24 hours were observed in C57BL/6 mice fed with 20% fructose water, 60% high fat diet, or the combination diet.Results:After 3 month feeding, all of the three energy- dense groups showed lipid accumulation in liver, respiratory quotient decrease, and energy expenditure increase compared with control group.Conclusion:Excess energy intake led to glucose metabolism injury and lipid metabolism increase. The pattern of energy metabolismwas changed fromglucose oxidation into lipid oxidation as energy supplier.