首页 | 本学科首页   官方微博 | 高级检索  
     


Phage release from biofilm and planktonic Staphylococcus aureus cells
Authors:Resch Alexandra  Fehrenbacher Birgit  Eisele Klaus  Schaller Martin  Götz Friedrich
Affiliation:Microbial Genetics, Universit?t Tübingen, D-72076 Tübingen, Germany.
Abstract:The ability of pathogenic staphylococci to form biofilms facilitates colonization and the development of chronic infections. Therapy is hampered by the high tolerance of biofilms towards antibiotic treatment and the immune system. We found evidence that lysogenic Staphylococcus aureus cells in a biofilm and in planktonic cultures spontaneously release phages into their surroundings. Phages were detected over a much longer period in biofilm cultures than in planktonic supernatants because the latter were degraded by secreted proteases. Phage release in planktonic and biofilm cultures was artificially increased by adding mitomycin C. Two morphologically distinct phages in the S. aureus strain used in this work were observed by electron microscopy. We postulate that phage-release is a frequent event in biofilms. The resulting lysis of cells in a biofilm might promote the persistence and survival of the remaining cells, as they gain a nutrient reservoir from their dead and lysed neighboring cells. This might therefore be an early differentiation and apoptotic mechanism.
Keywords:Bacteriophages    Biofilm    Lysogeny    Phage release    Electron microscopy
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号