AlCl3分光光度法测定荞麦种质资源中黄酮的研究

荞麦是黄酮的膳食来源之一。我国是世界保存荞麦种质资源最多的国家，为深入开发利用荞麦种质资源的功能成分，对AlCl3分光光度法测定荞麦种质资源黄酮含量的方法进行了研究，并对新收集的169份荞麦种质资源进行了测定评价。结果表明：①AlCl3分光光度法稳定性好，准确性和精密度高，偏差小，易于操作，适用于荞麦种质资源黄酮鉴定和评价；②苦荞种质资源黄酮含量的平均值、变异幅度、标准偏差分别为2．53％、1．94％～3．11％、0．2436，甜荞种质资源黄酮含量的平均值、变异幅度、标准差分别为0．13％、0．08％～0．21％、0．0273；苦荞种质资源黄酮含量的平均值约为甜荞的20倍。

Study on Flavone in Buckwheat Determined by Spectrophotometric Method

Flavone in buckwheat is a dietary source.In the world,the largest collection of buckwheat has been conserved in China.For developing and applying functional ingredient in buckwheat,the spectrophotometric method with AlCl3 was investigated to determine the flavone content in buckwheat germplasm,and flavone content of 169 accessions of buckwheat germplasm was evaluated.It indicated that this method possessed characteristics of well-stability,high-accuracy,low-deviation and easy-operating,so it was proved to be suitable for identification and evaluation of buckwheat germplasm.Also,it showed that the average,range and standard deviation of flavone content in tartary buckwheat germplasm was 2.53%,1.94 %-3.11%,0.2436,respectively;and that those of flavone content in common buckwheat germplasm was 0.13%,0.08%-0.21%,0.0273,respectively.The average content of tartary buckwheat germplasm appeared to be 20 times as high as that of common buckwheat germplasm.