Production of l-rhamnulose,a rare sugar,from l-rhamnose using commercial immobilized glucose isomerase |
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Authors: | Yeong-Su Kim Do-Yeon Kim |
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Institution: | 1. Division of Plant Resource Industry, Baekdudaegan National Arboretum, Bonghwa, South Korea;2. Department of Convergence Industrialization, International Ginseng and Herb Research Institute, Geumsan, South Korea |
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Abstract: | AbstractA commercial immobilized d-glucose isomerase from Streptomyces murines (Sweetzyme) was used to produce l-rhamnulose from l-rhamnose in a packed-bed reactor. The optimal conditions for l-rhamnulose production from l-rhamnose were determined as pH 8.0, 60?°C, 300?g L?1 l-rhamnose as a substrate, and 0.6?h?1 dilution rate. The half-life of the immobilized enzyme at 60?°C was 809?h. Under the optimal conditions, the immobilized enzyme produced an average of 135?g L?1 l-rhamnulose from 300?g L?1 l-rhamnose after 16 days at pH 8.0, 60?°C, and 0.6?h?1 dilution rate, with a productivity of 81?g/L/h and a conversion yield of 45% in a packed-bed reactor. |
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Keywords: | l-rhamnulose rare sugar glucose isomerase isomerization |
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