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Solid-State NMR Characterization of Gas Vesicle Structure |
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| Authors: | Astrid C. Sivertsen Marvin J. Bayro Marina Belenky Judith Herzfeld |
| Affiliation: | † Department of Chemistry, Brandeis University, Waltham, Massachusetts ‡ Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts § Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts |
| Abstract: | Gas vesicles are gas-filled buoyancy organelles with walls that consist almost exclusively of gas vesicle protein A (GvpA). Intact, collapsed gas vesicles from the cyanobacterium Anabaena flos-aquae were studied by solid-state NMR spectroscopy, and most of the GvpA sequence was assigned. Chemical shift analysis indicates a coil-α-β-β-α-coil peptide backbone, consistent with secondary-structure-prediction algorithms, and complementary information about mobility and solvent exposure yields a picture of the overall topology of the vesicle subunit that is consistent with its role in stabilizing an air-water interface. |
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