An extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (Brassica oleracea var. botrytis) |
| |
Authors: | N Delpierre J Boccon-Gibod |
| |
Institution: | (1) Lab. Amel. Pl. et Biotechnologies, ENITHP, 2 rue le Nôtre, 49045 Angers, France |
| |
Abstract: | Summary Protoplasts isolated from mesophyll tissues of cauliflower (Brassica oleraceavar. botrytis) were induced to divide in culture, with 2% of them producing calli. Upon transfer to a regeneration medium containing a low auxin/cytokinin balance (0.02mg/l 1-naphthaleneacetic and 2mg/l 6-benzylaminopurine), they displayed an extensive production of hairy roots before the regeneration of shoots. Negative effects of root differentiation on the subsequent caulogenesis by such calli were not observed, since 97% of the calli regenerated hairy roots and 93% gave shoots.Abbreviations NAA
1-naphthaleneacetic acid
- BAP
6-benzylaminopurine
- 2,4 D
2,4-dichlorophenoxyacetic acid
- GA3
gibberellic acid
- MES
2-N-morpholinoethane sulfonate |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|