| 黄山栾树体细胞胚的发生和组织学观察 |
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| 引用本文： | 冯大领,;李伟,;张洁,;李鹏涛,;李明,;赵书岗,;史宝胜,;彭伟秀.黄山栾树体细胞胚的发生和组织学观察[J].植物生理学通讯,2009(9):855-858. |
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| 作者姓名： | 冯大领 ;李伟 ;张洁 ;李鹏涛 ;李明 ;赵书岗 ;史宝胜 ;彭伟秀 |
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| 作者单位： | [1]河北农业大学生命科学学院,河北保定071001; [2]北京林业大学生物科学与技术学院,北京100083; [3]河北农业大学园林与旅游学院,河北保定071001 |
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| 基金项目： | 北京林业大学林木、花卉遗传育种教育部重点实验室开放基金（05-04）. |
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| 摘 要： | 黄山栾树无菌苗的节间和叶柄离体培养后，其体细胞胚发生的结果表明：节间愈伤组织可诱导产生体细胞胚，而叶柄愈伤组织则生根：节间愈伤组织诱导培养基为MS＋3．0mg．L～2,4．D＋0．5～3．0mg．L-1NAA；节间胚性愈伤组织诱导培养基为MS＋2．0nag．L-2,4-D；胚性愈伤组织转移到无植物生长调节剂的MS培养基上可发育成正常植株。组织学观察表明，体细胞胚在胚性愈伤组织中有的发生于愈伤组织表层细胞，有的发生在愈伤组织内部。黄山栾树体细胞胚的形成经历球形胚、心形胚、鱼雷胚和子叶胚几个阶段，这与合子胚的发育途径相似。
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| 关 键 词： | 黄山栾树 体细胞胚 胚性愈伤组织 组织学 |
Observation of Somatic Embryogenesis and Histology in Koelreuteria bipinnata Franch var. integrifoliola T. Chen |
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| Institution: | FENG Da-Ling, LI Wei, ZHANG Jie, LI Peng-Tao, LIMing, ZHAO Shu-Gang, SHI Bao-Sheng, PENG Wei-Xiu( 1College of Life Science, Agricultural University of HebeL Baoding, Hebei 071001, China; 2College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 3College of Landscape Architecture and Tourism, Agricultural University of Hebei, Baoding, Hebei 071001, China) |
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| Abstract: | Somatic embryogenesis of Koelreuteria bipinnata var. integrifoliola was studied in this paper. The results showed that somatic embryos were obtained from intersegment callus, and leafstalk callus produced root. Intersegment callus was MS medium＋3.0 mg.L-1 2,4-D＋0.5-3.0 mg-L-1 NAA. Embryonic callus was MS medium＋2.0 mg.L-1 2,4-D. Embryonic callus cells developed to normal seedlings when subcultured on MS medium without plant growth regulator. Histological observation revealed that the somatic embryos produced from cells of superficial callus or cells of inner callus and it successively developed through globular-shaped, heart-shaped, torpedo stages and cotyledon stages, similar to the development of zygotic embryo. |
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| Keywords: | Koelreuteria bipinnata var integrifoliola somatic embryo embryonic callus histology |
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