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TheRhizobium-legume symbiosis Two methods to discriminate between nodules and other root-derived structures
Authors:G. Truchet  S. Camut  F. de Billy  R. Odorico  J. Vasse
Affiliation:(1) Laboratoire de Biologie Moléculaire des Relations Plantes Microorganismes, INRA CNRS, Castanet-Tolosan
Abstract:Summary Two methods have been developed in order to discriminate between lateral roots, nodules and root-derived structures which exhibit both root and nodule histological features and which can develop on legumes inoculated with certainRhizobium mutants. The first method, known as the ldquoclearing methodrdquo, allows the observation by light microscopy of cleared undissected root-structures. The second, known as the ldquoslicing methodrdquo, is a complementary technique which provides a greater degree of structural information concerning such structures. The two methods have proved invaluable in defining unequivocally the nature of the interaction between a rhizobial strain and a legume host.
Keywords:Legumes  Nitrogen fixation  Nodulation  Rhizobium  Symbiosis
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