Isolation of infective and effective Frankia strains from root nodules of Alnus acuminata (Betulaceae) |
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Authors: | Carú M Becerra A Sepúlveda D Cabello A |
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Institution: | (1) Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile;(2) Instituto Multidisciplinario de Biología Vegetal (IMBIV), Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina;(3) Departamento de Silvicultura, Facultad Ciencias Forestales, Universidad de Chile, Chile |
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Abstract: | Two Frankia strains were isolated from root nodules of Alnus acuminata collected in the Tucumano-oranense forest, Argentina. Monosporal cultures were obtained by plating a spore suspension of
each strain and isolating a single colony. The strains (named AacI and AacIII) showed branched mycelia with polymorphic sporangia
and NIR-vesicles. They differed in their ability to use carbon sources: the AacI strain grew well on pyruvate, while the AacIII
strain grew on mineral medium supplemented with glucose or, alternatively, with sucrose. The two strains were sensitive to
oleandomycin, erythromycin, kanamycin, penicillin G, streptomycin and chloramphenicol at 5 μg/ml. The AcIII strain exhibited
a moderate resistance to rifampicin, ampicillin and vancomycin. The nitrogenase activity in vitro of the strains was significantly higher in basal medium without nitrogen than that determined in the presence of ammonium
chloride. Both strains were infective on seedlings of Alnus glutinosa, inducing an approximately similar percentage of nodulated plants (80%), although strain AacIII produced a higher number
of nodules per plant (≤15) than strain AacI (≤6). They were also effective for nitrogen fixation in planta, determined by the acetylene reduction assay.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | Alnus acuminata Frankia nitrogen fixation symbiosis |
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