Biotransformation of hydroquinone to arbutin in plant <Emphasis Type="Italic">in vitro</Emphasis> cultures — preliminary results |
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Authors: | Ewa Skrzypczak-Pietraszek Agnieszka Szewczyk Agata Piekoszewska Halina Ekiert |
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Institution: | (1) Chair and Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, 9 Medyczna Street, 30-688 Kraków, Poland |
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Abstract: | Cells from Echinacea purpurea (L.) Moench. (Asteraceae), Exacum affine Balf. f. (Gentianaceae), Melittis melissophyllum L. (Lamiaceae), Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. (Rutaceae) agitating cultures perform a biotransformation reaction on exogenously supplied hydroquinone into its β-D-glucoside — arbutin,
product with valuable medicinal and cosmetic properties. The maximum content of arbutin (determined by HPLC) in the biomass
from investigated cultures is 4.01; 3.44; 1.79; 2.48 and 5.07 g/100 g d.w., respectively. Nothing but Ammi majus L. (Apiaceae) cultures contain trace amounts of the product. Arbutin is accumulated in cells; it is occasionally found in media only in
vestigial amounts. In most of the investigated cultures the efficiency of the biotransformation process is about 60 %. |
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Keywords: | Echinacea purpurea Exacum affine glucosylation Melittis melissophyllum Ruta graveolens and Ruta graveolens ssp divaricata |
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