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DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools |
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| Authors: | Niederhauser Barbara Siivonen Joonas Määttä Juha A Jänis Janne Kulomaa Markku S Hytönen Vesa P |
| Affiliation: | a Institute of Biomedical Technology, University of Tampere and Tampere University Hospital, Tampere, Finland b BioMediTech, Tampere, Finland c Department of Chemistry, University of Eastern Finland, Joensuu, Finland |
| Abstract: | Avidins represent an interesting group of proteins showing high structural similarity and ligand-binding properties but low similarity in primary structure. In this study, we show that it is possible to create functional chimeric proteins from the avidin protein family when applying DNA family shuffling to the genes of the avidin protein family: avidin, avidin related gene 2 and biotin-binding protein A. The novel chimeric proteins were selected by phage display biopanning against biotin, and the selected enriched proteins were characterized, displaying diverse features distinct from the parental genes, including binding to cysteine. |
| Keywords: | AVD, chicken avidin AVR, avidin related protein BBP-A, biotin-binding protein A BTN, biotin A/A2-1, chimeric protein containing sequence from avidin and AVR2 clone number 1 A/B-1, chimeric protein containing sequence from AVD and BBP-A clone number 1 |
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