Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

Authors:	Richardson Leisha J Kaestli Mirjam Mayo Mark Bowers Jolene R Tuanyok Apichai Schupp Jim Engelthaler David Wagner David M Keim Paul S Currie Bart J

Affiliation:	^a Menzies School of Health Research, Charles Darwin University, Darwin, Australia ^b TGen, Flagstaff, USA ^c Northern Arizona University, Flagstaff, USA ^d Infectious Diseases Department, Northern Territory Clinical School, Royal Darwin Hospital, Darwin, Australia

Abstract:	Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Keywords:	Melioidosis Burkholderia pseudomallei Diagnostic Clinical specimen DNA extraction Real-time PCR
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