Immunological and chemical characterization of hamster brain polyribosomes-cytomatrix complexes |
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| Authors: | Ihssane Bouhtiauy Yassin Choukri Christian Turpin Didier Gauthier |
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| Affiliation: | (1) Départment de chimie et biochimie, Université de Moncton, E1A 3E9 Moncton, N-B., Canada;(2) Present address: Hopital Maisonneuve, H1T 2M4 Montréal, Qc, Canada |
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| Abstract: | We have studied the cytoskeletal nature of a brain subcellular fraction previously shown to contain polyribosomes. We have identified the major proteins of this fraction by electrophoretic comparison to a standard cytoskeletal fraction and by immunodetection. These methods have shown the presence of actin, glial fibrillary acidic protein, and neurofilament triplet proteins. We have also studied the effect of various ions and nonionic detergents on the stability of this structure. It was stable in presence of Triton X-100 up to 2% but disrupted by 200 mM K+ acetate.Abbreviations CMT cytomatrix - CSK cytoskeleton - DOC sodium deoxycholate - DTT dithiothreitol - EGTA ethylenglycolbis ( -Ether)-N,N-N -N-Tetraacetic Acid - GFAP glial fibrillary acidic protein - PR polyribosome - PRCMC polyribosomes-cytomatrix complex |
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| Keywords: | Cytoskeleton cytomatrix polyribosomes protein synthesis |
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