| 肌间隙移植明胶海绵获取成体干细胞 |
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| 引用本文： | 武晓云,王世立,牟忠祥.肌间隙移植明胶海绵获取成体干细胞[J].中国生物工程杂志,2011,31(6):6-11. |
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| 作者姓名： | 武晓云 王世立 牟忠祥 |
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| 作者单位： | 1. 卫生部生物技术药物重点实验室 山东省医药生物技术研究中心 济南 250062;
2. 山东省中医药大学附属医院 济南 250011 |
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| 基金项目： | 国家自然科学基金(30940065,30571766); 山东省自然科学基金(Y2008C06)资助项目 |
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| 摘 要： | 通过移植生物材料到小鼠体内而建立一种新的干细胞获取方法.移植明胶海绵到小鼠后肢的大腿内侧肌间隙,12天后分离明胶海绵中的迁移细胞.海绵来源干细胞形态、增殖能力、多向分化能力与骨髓源干细胞相似,但海绵中成纤维细胞集落形成单位的比例(82.2±10.6/105)远远高于骨髓中的比例(43.7±7.4/106)(P＜0.05...
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| 关 键 词： | 明胶海绵 成体干细胞 细胞培养 分化 |
| 收稿时间： | 2010-11-23 |
| 修稿时间： | 2011-01-19 |
Adult Stem Cells Harvested by Implanting Gelatin Sponges into Spatium Intermusculare |
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| WU Xiao-yun,WANG Shi-li,MU Zhong-xiang.Adult Stem Cells Harvested by Implanting Gelatin Sponges into Spatium Intermusculare[J].China Biotechnology,2011,31(6):6-11. |
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| Authors: | WU Xiao-yun WANG Shi-li MU Zhong-xiang |
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| Institution: | WU Xiao-yun1 WANG Shi-li1 MU Zhong-xiang2(1 Key Laboratory for Biotech-Drugs Ministry of Health Shandong Medicinal Biotechnology Center,Jinan 250062,China)(2 Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,China) |
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| Abstract: | A new method to harvest stem cells was established by implanting biomaterials into mice. Gelatin Sponges (GS) were implanted into the spatium intermusculare of mice hind limbs. A large number of migrating cells were isolated from the transplanted biomaterials at 12 days after implanting. The characteristics of adherent cells were similar to that of bone marrow (BM) stem cells, including morphology, proliferation potential and multilineage differentiation capacity. The frequency of CFU-F in GS (82.2±10.6/105) was much higher than in BM (43.7±7.4/106) (P<0.05). In additional, BM transplantation demonstrated that stem cells in the GS originated from the PB. Moreover, it is possible for autologous cell therapy, so this method may be a promising alternative for the clinical application. |
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| Keywords: | Gelatin Sponge Adult stem cells Cell culture Differentiation |
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