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| 厚壳贻贝Mytilus coruscus足丝盘及足丝的多巴分析 | |
| 引用本文： | 李楠楠,石戈,杨宵旭,王智平,廖智. 厚壳贻贝Mytilus coruscus足丝盘及足丝的多巴分析[J]. 中国生物化学与分子生物学报, 2011, 0(2): 148-153 |
| 作者姓名： | 李楠楠 石戈 杨宵旭 王智平 廖智 |
| 作者单位： | 浙江海洋学院海洋科学学院海洋生物资源及分子工程实验室;湖南师范大学生命科学学院蛋白质化学及发育生物学教育部重点实验室; |
| 基金项目： | Supported by Research and Agricultural Project of Science and Technology Department of Zhejiang Province(No.2008C22026); Key Project of Education Department of Zhejiang Province(No.20070430)~~ |
| 摘 要： | 多巴(3,4-1-dihydroxyphenylalanine,DOPA)是贻贝足丝粘附蛋白中的一种特殊的氨基酸,由酪氨酸经羟化后生成,与贻贝足丝粘附蛋白的强粘附性能具有直接联系.目前,已鉴定的多种贻贝足丝蛋白序列中均发现有不同含量的DOPA存在.蛋白中DOPA的定量检测对于了解DOPA在蛋白粘附中的作用以及粘附蛋白的... |
| 关 键 词： | Mytilus corusus 多巴(DOPA) 足丝 足丝纤维 足丝盘 |
| 收稿时间： | 2010-09-16 |
Detection of Dihydroxyphenylalanine in Native Foot Proteins from Mytilus coruscus Byssus |
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| LI Nan-Nan),SHI Ge),YANG Xiao-Xu),WANG Zhi-Ping),LIAO Zhi) Laboratory of Marine Biological Resources , Molecular Engineering,College of Marine Science,Zhejiang Ocean University,Zhoushan ,Zhejiang,China,) Key Laboratory of Protein Chemistry , Developmental Biology of Ministry of Education,College of Life Sciences,Hunan Normal University,Changsha ,China). Detection of Dihydroxyphenylalanine in Native Foot Proteins from Mytilus coruscus Byssus[J]. Chinese Journal of Biochemistry and Molecular Biology, 2011, 0(2): 148-153 | |
| Authors: | LI Nan-Nan) SHI Ge) YANG Xiao-Xu) WANG Zhi-Ping) LIAO Zhi) Laboratory of Marine Biological Resources Molecular Engineering College of Marine Science Zhejiang Ocean University Zhoushan Zhejiang China ) Key Laboratory of Protein Chemistry Developmental Biology of Ministry of Education College of Life Sciences Hunan Normal University Changsha China) |
| Affiliation: | LI Nan-Nan1),SHI Ge1),YANG Xiao-Xu2),WANG Zhi-Ping1),LIAO Zhi1)*(1) Laboratory of Marine Biological Resources and Molecular Engineering,College of Marine Science,Zhejiang Ocean University,Zhoushan 316004,Zhejiang,China,2) Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education,College of Life Sciences,Hunan Normal University,Changsha 410081,China) |
| Abstract: | The amino acid 3,4-l-dihydroxyphenylalanine (DOPA) can be detected in a variety of foot proteins in mussel. The quantification of DOPA in proteins is essential to understand its role in mussel adhesion. DOPA can be detected with considerable sensitivity with nitroblue tetrazolium /Glycinate(NBT/Glycinate) staining. The Mytilus coruscus byssal proteins were experimented for the detections of the DOPA content,protein distribution,and adhesive ability of foot proteins from thread and plaque with electrophoresi... |
| Keywords: | Mytilus coruscus 3 4-l-dihydroxyphenylalanine (DOPA) byssus thread plaque |
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