| 利用荧光共振能量转移技术监测高通量低能量激光诱导细胞凋亡过程中caspase-3活性的动态变化 |
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| 引用本文： | 吴胜男,邢达. 利用荧光共振能量转移技术监测高通量低能量激光诱导细胞凋亡过程中caspase-3活性的动态变化[J]. 激光生物学报, 2007, 16(4): 400-404 |
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| 作者姓名： | 吴胜男 邢达 |
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| 作者单位： | 华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,中国,广州,510631;华南师范大学激光生命科学研究所暨激光生命科学教育部重点实验室,中国,广州,510631 |
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| 基金项目： | 国家自然科学基金项目(60378043,30470494)，广东省自然科学基金项目(015012,04010394)，广东省科技计划项目(2004B10401011) |
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| 摘 要： | 荧光共振能量转移可用于对生物大分子之间的距离进行定性、定量检测。应用荧光共振能量转移技术对高通量低能量激光诱导肺腺癌细胞凋亡过程中caspase-3的激活过程进行实时动态监测。实验结果表明:高通量低能量激光可以诱导肺腺癌细胞(human lung adenocarcinoma cell,ASTC-a-1)凋亡。同时荧光共振能量转移技术是一个有效的监测caspase-3在凋亡过程中活性动态变化的方法。
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| 关 键 词： | 荧光共振能量转移 低能量激光 细胞凋亡 |
| 文章编号： | 1007-7146（2007）04-0400-05 |
| 收稿时间： | 2007-03-26 |
| 修稿时间： | 2007-03-26 |
Monitoring Dynamics of caspase-3 Activity in Living Cells Using FRET Technique during Apoptosis Induced by High Fluence LPLI |
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| WU Sheng-nan,XING Da. Monitoring Dynamics of caspase-3 Activity in Living Cells Using FRET Technique during Apoptosis Induced by High Fluence LPLI[J]. ACTA Laser Biology Sinica, 2007, 16(4): 400-404 |
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| Authors: | WU Sheng-nan XING Da |
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| Affiliation: | MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510631, Guangdong, China |
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| Abstract: | Fluorescence resonance energy transfer(FRET) can be used for measuring the distance between the molecules in living cells.In our study,We use FRET technique to measure the dynamics of caspase-3 activity in living cells during apoptosis induced by high fluence low-power laser irradiation(LPLI).The result showed that high fluence LPLI could induce apoptosis of ASTC-a-1 cells and FRET technique was an effective method to monitor dynamics of caspase-3 activity in apoptosis. |
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| Keywords: | fluorescence resonance energy transfer(FRET) low-power laser irradiation(LPLI) apoptosis |
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