Detection of squirrel poxvirus by nested and real-time PCR from red (<Emphasis Type="Italic">Sciurus vulgaris</Emphasis>) and grey (<Emphasis Type="Italic">Sciurus carolinensis</Emphasis>) squirrels |
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| Authors: | Janus W Atkin Alan D Radford Karen P Coyne Jenny Stavisky Julian Chantrey |
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| Institution: | (1) Department of Veterinary Pathology, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK;(2) Department of Veterinary Clinical Sciences, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK |
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| Abstract: | Background Squirrel poxvirus (SQPV) is highly pathogenic to red squirrels (Sciurus vulgaris), and is a significant contributing factor to the local extinction of the species in most parts of England and Wales, where
infection is endemic in Eastern grey squirrel (Sciurus carolinensis) populations. Although a nested PCR assay has been used successfully to study the epidemiology of SQPV, samples have a long
processing time and the assay is not quantifiable. |
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