Simultaneous determination of thioridazine and its S-oxidized and N-demethylated metabolites using high-performance liquid chromatography on radially compressed silica |
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| Authors: | Clinton D. Kilts Kennerly S. Patrick George R. Breese Richard B. Mailman |
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| Affiliation: | Clinical Psychopharmacology Laboratory, Department of Psychiatry, Box 3870, Duke University Medical Center, Durham, NC 27710 U.S.A.;Biological Sciences Research Center, University of North Carolina, Chapel Hill, NC 27514 U.S.A.;Departments of Psychiatry and Pharmacology and the Biological Sciences Research Center, University of North Carolina, Chapel Hill, NC 27514 U.S.A. |
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| Abstract: | A method for simultaneously quantifying thioridazine, northioridazine, thioridazine-2-sulfoxide, thioridazine-2-sulfone and thioridazine-5-oxide in serum and plasma is described. Following solvent extraction these compounds were separated by high-performance liquid chromatography on radially compressed silica gel and detected by UV absorbance at 254 nm. Chromatography time is less than 7 min. The relative retention of these compounds as a function of the methanol and methylamine content of the mobile phase is discussed. Practical limits of detection, based upon an assayed plasma or serum volume of 1 ml, were 20 ng/ml for thioridazine-5-oxide and 10 ng/ml for the other compounds. The coefficient of variation for all compounds was less than 13%. The method is compared with more conventional high-performance liquid chromatographic and gas chromatographic methodology. |
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