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Resveratrol inhibits firefly luciferase
Authors:Bakhtiarova Adel  Taslimi Paul  Elliman Stephen J  Kosinski Penelope A  Hubbard Brian  Kavana Michael  Kemp Daniel M
Affiliation:Diabetes and Metabolism Disease Area, Novartis Institutes for BioMedical Research, 100 Technology Square, Cambridge, MA 02139, USA.
Abstract:
The potential therapeutic value of resveratrol in age-related disease settings including cancer, diabetes, and Alzheimer's has emerged from a rapidly growing body of experimental evidence. Protection from oxidative stress appears to be a common feature of resveratrol that may be mediated through SirT1, though more specific molecular mechanisms by which resveratrol mediates its effects remain unclear. This has prompted an upsurge in cell-based mechanistic studies, often incorporating reporter assays for pathway elucidation in response to resveratrol treatment. Here, we report that resveratrol potently inhibits firefly luciferase with a K(i) value of 2microM, and caution that this confounding element may lead to compromised data interpretation.
Keywords:Resveratrol   Firefly luciferase   SirT1   Therapeutic   Drug   Cancer   Neurodegeneration
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