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An approach to study the evolution of theDrosophila 5S ribosomal genes using P-element transformation |
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| Authors: | Marie-Laure Samson Maurice Wegnez |
| Institution: | (1) Laboratoire d’Embryologie Moléculaire et Expérimentale, Unité de Recherche Associée 25, Centre National de la Recherche Scientifique, Université Paris 11, Batiment 445, 91405 Orsay;(2) Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique, 91190 Gif-sur-Yvette, France;(3) Present address: Department of Molecular Biology, Massachusetts General Hospital, 02114 Boston, Massachusetts, USA |
| Abstract: | Summary The P-element-mediated gene transfer system was used to introduceDrosophila teissieri 5S genes into theDrosophila melanogaster genome. Eight transformedD. melanogaster strains that carryD. teissieri 5S mini-clusters consisting of 9–21 adjacent 5S units were characterized. No genetic exchanges betweenD. melanogaster andD. teissieri 5S clusters were detected over a 2-year survey of the eight strains. The occurrence of small rearrangements within theD. melanogaster 5S cluster was demonstrated in one of the transformed strains. |
| Keywords: | Drosophila melanogaster Drosophila teissieri 5S gene Concerted evolution P-element transformation |
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