兔海水淹溺型肺水肿组织NKA活性与动脉血气的相关性分析

目的:观察兔海水淹溺型肺水肿组织钠-钾ATP酶(Na~ -K~ -ATPase)活性变化与动脉血气变化的关系,探讨钠-钾ATP酶在海水淹溺肺水肿形成中的重要作用,为临床治疗提供实验依据。方法:将30只新西兰兔随机分为对照组(5只)及淹溺组(25只),淹溺组根据观察时间段分为10′、30′、60′、90′、120′组(每组5只),采用气管切开插入塑料导管、向气管内灌海水3 mL/kg、双肺自主通气的方法进行兔海水淹溺肺水肿模型复制。于各观察时间点进行血气分析,并采集肺组织标本,对肺组织匀浆钠-钾ATP酶进行测定。结果:兔海水淹溺肺水肿各时间点钠-钾ATP酶活性降低,动脉血气显示低氧和酸中毒,钠-钾ATP酶活性变化与动脉血气主要指标变化具有线性回归关系(P<0.01)。结论:海水淹溺型肺水肿组织NKA活性与动脉血气的具有相关性,钠-钾ATP酶在海水淹溺肺水肿发生发展中具有重要作用。

The Correlative Analysis of Lung Na+-K+-ATPase Activity and Arterial Blood Gas in PE-SWD of Rabbits

Objectives:To observe the relationship between the change of Na~ -K~ -ATPase activity and arterial blood gas in PE-SWD of rabbits,explore the significant effect in PE-SWD by Na~ -K~ -ATPase and to provide experimental evidence for clinical treat- merit.Methods:30 New Zealand rabbits were randomly divided into the control group (n=5) and the drowning group(n=25),according to different drowning times,the latter were divided into 10min,30min,60min,90min and 120min group (5 rabbits each group).The pulmonary edema model was established with inserting plastic tube into the wachea of rabbits,and seawater (3mL/kg) was poured into air tube with both lungs auto ventilated of the process when drowning.Arterial blood gas and the Na~ -K~ -ATPase activity of lung homo- genate in different drowning times were determined.Results:The Na~ -K~ -ATPase activity of lung homogenate of drowning groups were markedly lower than that of the control group.Severe hypoxia and acidosis were showed in arterial blood gas after drowning.Linear re- gression were determined between the main index of arterial blood gas and the Na -K~-ATPase activity of lung homogenate (p<0.01). Conclusions:Na~ -K~ -ATPase activity of lung homogenate has relationship with arterial blood gas in PE-SWD.Na~ -K~ -ATPase play a great role in PE-SWD.