沙漠干热环境下猪腹部肠管火器伤后肝脏功能和形态的变化

目的:观察沙漠干热环境下猪腹部火器伤肠管穿透后肝脏功能和形态的变化。方法:沙漠干热环境组和常温环境组各健康长白仔猪42头随机等分为对照组和伤后1h、2h、4h、8h、12h和24h组,实验组建立腹部火器伤肠管穿透模型后,分别测定各组动物血清中AST水平,并与对照组比较。在光镜下观察各组肝脏组织学变化,电镜下观察肝脏超微结构改变。结果:伤后各组血清AST水平均高于对照组,并于伤后2h出现第1个高峰,沙漠干热环境组和常温环境组分别于伤后8h和12h出现第2个高峰。沙漠干热组和常温组分别于伤后4h和8h开始出现肝细胞的坏死和超微结构的明显变化。结论:沙漠干热环境下腹部肠管火器伤后肝脏结构和功能损伤的发生均较常温组提前,提示在沙漠干热环境下腹部火器伤更要及早对肝损伤采取相应的保护措施。

Changes of Hepatic Morphology and Function in Pigs of Intestinal Perforations Due to Abdominal Firearm Wound in Dry-heat Environment of Desert

Objective:To investigate the changes of hepatic function and morphology in pigs of intestinal perforations due to ab- dominal firearm wound in dry-heat environment of desert.Methods:84 Chang-Bai piglets were divided equally into normal temperature environment group and dry-heat desert environment group.Each group was equally divided into 7 groups randomly:control group and lhour group,2 hour group,4 hour group,8 hour group,12 hour group and 24 hour group after injure.After intestinal perforations model of abdominal firearm wound was established,serum AST levels of wounded groups were determined and compared with the control.The alterations of hepatic tissue were observed under light microscope and the ultrastructural changes of liver were observed under electron microscope.Results:Serum AST level in wounded groups was significantly increased in comparison to the control group.Serum AST level of the normal temperature group reached peak after 2h and 12h of wounding respectively,and that of the dry-heat environment group reached peak after 2h and 8h of injure respectively.Hepatocyte necrosis and hepatic ultrastructural injuries were obviously ob- served in 4h group of dry-heat desert environment group and in 8h group of normal temperature environment group respectively.Conclu- sions:Hepatic functional and morphological injury induced by intestinal perforations due to abdominal firearm wound in dry-heat desert environment group was earlier evoked than in normal temperature environment group.It is suggested that protective measure for liver in- jury should be adopted in abdominal firearm wound of dry-heat desert environment as early as possible.