| 辣椒子叶原生质体分离条件的研究 |
| |
| 引用本文： | 何晓明,王哲之.辣椒子叶原生质体分离条件的研究[J].西北植物学报,1997,17(1):112-117. |
| |
| 作者姓名： | 何晓明 王哲之 |
| |
| 作者单位： | [1]华南师范大学生物系植物生理学博士后流动站 [2]陕西师范大学生物系 |
| |
| 基金项目： | 高等学校博士学科点专项研究基金 |
| |
| 摘 要： | 以不同基因型的辣椒子叶为供体组织进行辣椒原生质体分离条件的研究，结果表明：幼龄子叶的原生质体产量与活力均高于老龄子叶；酶解过程中酶液渗透压、酶液浓度、酶解时间均对原生质体分离效果产生重要影响。对于辣椒子叶原生质体，最佳分离条件为酶液甘露醇浓度０．５ｍｏｌ／Ｌ，纤维素酶Ｃｅｌｌｕｌａｓｅ Ｏｎｚｕｋａ Ｒ－１０ １．５，果胶酶Ｍａｃｅｒｏｚｙｍｅ Ｒ－１０ ０．６％，酶解时间８－１０ｈ。不同基因型辣
|
| 关 键 词： | 辣椒 子叶 原生质体 分离 |
STUDY ON PROTOPLAST ISOLATION FROM COTYLEDONS OF PEPPER |
| |
| He Xiaoming and Wang Ming.STUDY ON PROTOPLAST ISOLATION FROM COTYLEDONS OF PEPPER[J].Acta Botanica Boreali-Occidentalia Sinica,1997,17(1):112-117. |
| |
| Authors: | He Xiaoming and Wang Ming |
| |
| Abstract: | The effects of fators on protoplast isolation from pepper (Capst 'um annuum L.)were studied. The results showed that the protoplasts with higher yield and viabilitywere isolated from young cotyledons. The important effects of osmoticum,enzyme concentration of enzymesolution and incubation period were observed.Tht enzyme solutionwith 0. 5 mol/L manntal, 1. 5 % Cellulase Onzuka R-10 and 0. 5 % Wacerozyme R-10,and 8-10 h incubation period were most suitable for protoplast isolation from peppercotyledons. No significant difference on yield and viability of protoplast was found between different genetypes of pepper. |
| |
| Keywords: | pepper(Capsicum annuum L ) cotyledon protoplast isolation |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《西北植物学报》浏览原始摘要信息 |
| 点击此处可从《西北植物学报》下载免费的PDF全文 |
