Rapid methods of detecting the target molecule in immunohistology using a bioluminescence probe |
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| Authors: | Chun Wu Ke‐Yong Wang Xin Guo Masanori Sato Michitaka Ozaki Shyohei Shimajiri Yoshihiro Ohmiya Yasuyuki Sasaguri |
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| Institution: | 1. Health Research Institute, National Institute of AIST, , Osaka, Japan;2. Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, , Kita‐kyusyu, Japan;3. Graduate School of Medicine, Hokkaido University, , Sapporo, Japan;4. Bioproduction Research Institute, National Institute of AIST, , Ibaraki, Japan |
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| Abstract: | We demonstrate a novel rapid direct detection method for immunohistochemistry, using a bioluminescent probe. An anti‐CEA antibody‐fused far‐red bioluminescent protein can monitor the accumulation of this type of probe in tumour tissues. The bimodal spectrum (λmax = 460 and 675 nm) of this bioluminescent probe is extremely stable under different conditions of pH and ion concentration. The sensitivity of our bioluminescent labelling was at the same level of enzymatic labelling, e.g. peroxidase, as an indirect system. Our novel technique is simple and can shorten the pretreatment time of paraffin sections to around 30 min. The utility of our bioluminescent labelling covers all imaging in vitro, in vivo and ex vivo, suggesting that our antibody‐fused bioluminescent probe has the potential to detect tumour antigens with a high sensitivity in routine immune histological examinations. Copyright © 2012 John Wiley & Sons, Ltd. |
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| Keywords: | bioluminescent probe immunohistochemistry luciferase luciferin disease‐specific antigens |
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