Antioxidant properties of mesenchymal stem cells against oxidative stress in a murine model of colitis |
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| Authors: | Fabiany da Costa Gonçalves Mateus Grings Natália Schneider Nunes Fernanda Otesbelgue Pinto Tuane Nerissa Alves Garcez Fernanda Visioli Guilhian Leipnitz Ana Helena Paz |
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| Institution: | 1.Experimental Research Center,Hospital de Clínicas de Porto Alegre,Porto Alegre,Brazil;2.Graduate Program in Gastroenterology and Hepatology Sciences,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;3.Department of Biochemistry, Instituto de Ciências Básicas da Saúde,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil;4.Oral Pathology Department, School of Dentistry,Universidade Federal do Rio Grande do Sul,Porto Alegre,Brazil |
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| Abstract: | ObjectiveTo investigate the effects of oxidative stress injury in dextran sulfate sodium (DSS)-induced colitis in mice treated with mesenchymal stem cells (MSC).ResultsMice exposed to oral administration of 2% DSS over 7 days presented a high disease activity index and an intense colonic inflammation. Systemic infusion of MSC protected from severe colitis, reducing weight loss and diarrhea while lowering the infiltration of inflammatory cells. Moreover, toxic colitis injury increased oxidative stress. Administration of DSS decreased reduced glutathione (GSH) and superoxide dismutase (SOD) activity, and increased thiobarbituric acid-reactive substances levels in the colon. No alteration was found in catalase (CAT) and glutathione peroxidase (GPx) activity. Otherwise, MSC transplantation was able to prevent the decrease of GSH levels and SOD activity suggestive of an antioxidant property of MSC.ConclusionThe oxidative stress is a pathomechanism underlying the pathophysiology of colitis and MSC play an important role in preventing the impairment of antioxidants defenses in inflamed colon. |
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