| 里氏木霉液体发酵产纤维素酶的研究 |
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| 引用本文： | 谭宏,谢小保.里氏木霉液体发酵产纤维素酶的研究[J].工业微生物,1996,26(1):7-11. |
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| 作者姓名： | 谭宏 谢小保 |
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| 作者单位： | 广东省微生物研究所，广东省微生物研究所，广东省微生物研究所，广东省微生物研究所 广州 510070，广州 510070，广州 510070，广州 510070 |
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| 摘 要： | 在摇瓶试验基础上,采用里氏木霉(Trichoderma reesei)HC-415菌株进行5L自控罐产纤维素酶深层发酵试验。在通气量为 0.2—0.6vvm、搅拌速度为 400r/min、发酵液pH控制在5.8—6.1的条件下,发酵液的羧甲基纤维素(CMC)酶酶活最高为325.0mg糖/ml,滤纸糖酶(FPA)酶活最高达17.9mg糖/ml。发酵周期为108h。所得冻干纤维素酶粉CMC酶活最高3111IU/g,FPA最高135IU/g ,对发酵液得率平均6.7g/L。酶活总收率CMC酶活平均78.2％,FPA酶活平均73.5％。
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| 关 键 词： | 纤维素酶 里氏木霉 深层发酵 |
Production of Cellulase Using Submerged Fermentation by Trichoderma reesei HC-415 |
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| Tan Hong,Xie Xiaobao,Mo Yong,Den Jinghua.Production of Cellulase Using Submerged Fermentation by Trichoderma reesei HC-415[J].Industrial Microbiology,1996,26(1):7-11. |
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| Authors: | Tan Hong Xie Xiaobao Mo Yong Den Jinghua |
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| Abstract: | Submerged fermentation of cellulase by Trichoderma reesei HC-415 was carried out in a 5L fermentor. The optimum ventilation quantity and agitation speed were 0.2-0.6 vvm and 400r/min respectively. The highest CMC and FP enzyme activities were 325.0 U/ml and 17.9U/ml. The fermentation cycle was 108 hours. The highest CMC and FP enzyme activities of cellulase powder obtained by freeze drying were 3111 IU/g and 135 IU/g. The average yield from fermentation broth was 6.7g/L. The average recovery of CMC and FP enzyme activities were 78.2% and 73.7% respectively. |
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| Keywords: | Trichoderma reesei Cellulase Submerged fermentation |
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