Biodistribution and toxicity assessment of copper nanoparticles in the rat brain |
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| Affiliation: | 1. Department of Environment and Health, Italian National Institute for Health, Viale Regina Elena 299, 00161 Rome, Italy;2. Department of Biomedical Sciences—Histology, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy;3. Department of Medical Surgical and Experimental Sciences, Ophthalmology Unit, University of Sassari, Viale San Pietro 43/C, 07100 Sassari, Italy;4. I.S.R.C.T.––International Society for Research on Cadmium Toxicity, Viale San Pietro 43/B, 07100 Sassari, Italy;1. Laboratory of Microscopy Applied to Reproduction, Department of Histology, Embryology and Cell Biology, Institute of Biological Sciences, Federal University of Goiás, Goiania, Goias 74001970, Brazil;2. Laboratory of Microscopy and Microanalysis, Department of Biology, University Estadual Paulista – UNESP, Rua Cristóvão Colombo, 2265, São José do Rio Preto, Sao Paulo 15054000, Brazil;3. Laboratory of Molecular and Biochemistry Pharmacology, Department of Pharmacology, Institute of Biological Sciences, Federal University of Goiás, Goiania Goias 74001970, Brazil;1. Department of Family Planning Technical Service, Jining Maternal and Child Health Family Planning Service Center, No.12, Gongxiao Road, Rencheng District, Jining City, Shandong Province, 272000, China;2. Department of Laboratory, Jining Maternal and Child Health Family Planning Service Center, No.12, Gongxiao Road, Rencheng District, Jining City, Shandong Province, 272000, China;1. Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, Changsha, 410078, China;2. Center of Clinical Pharmacology, the Third Xiangya Hospital, Central South University, Changsha, 410013, China;3. Environmental Science and Engineering, College of Resource and Environment, Hunan Agricultural University, Changsha, 410128, China;4. Department of Occupational and Environmental Health, Huazhong University of Science and Technology, Wuhan, 430030, China;5. Department of Social Medicine and Health Management, Xiangya School of Public Health, Central South University, Changsha, 410078, China;6. Hunan Occupational Disease Prevention and Control Institute, Changsha, 410007, China;7. Public Health College, Kunming Medical University, Kunming, 650500, China;8. Center for Environment and Health in Water Source Area of South-to-North Water Diversion, Hubei University of Medicine, Shiyan, 442000, China;9. Department of Dermatology, Xiangya Hospital, Central South University, Changsha, 410008, China;1. Laboratory of Oxidative Stress, Centre for Interdisciplinary Research, The John Paul II Catholic University of Lublin, Poland;2. Institute of Hematology and Transfusion Medicine, Warsaw, Poland;3. Clinic of Haematooncology and Bone Marrow Transplantation, Medical University, Lublin, Poland |
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| Abstract: | ![]()
AimsThe increase in the usage of copper nanoparticles (Cu NPs) in the industrial and medical fields has raised concerns about their possible adverse effects. The present study aims to investigate the potential adverse effects of Cu NPs on the brain of adult male Wistar rats through the estimation of some oxidative stress parameters and acetylcholinesterase (AChE) activity.Basic proceduresCu NPs were prepared and characterized using different techniques: Dynamic Light Scattering, X-Ray Diffraction, Transmission and Scanning Electron Microscopy, Fourier transform Infrared Spectroscopy, in addition to Energy Dispersive X-ray Spectroscopy. Rats were divided into two groups: Cu NPs-treated group (IV injected with 15 mg/kg ˷ 13 nm Cu NPs for 2 successive days) and a control group (injected with saline). Rats of the 2 groups were decapitated simultaneously after 48 h of the last injection. The Cu content in different brain areas was analyzed using inductively coupled plasma mass spectrometry. Moreover, the effect of Cu NPs on brain edema was evaluated. The behavior of rats in an open-field was also examined 24 h post the last injection.Main findingsSignificant increases of Cu content in the cortex, cerebellum, striatum, thalamus and hippocampus were found. Moreover, Cu NPs lead to the induction of oxidative stress condition in the thalamus, hypothamaus and medulla. In addition, Cu NPs induced significant increases in AChE activity in the medulla, hippocampus, striatum besides midbrain. Cu NPs-injected rats showed also decreased exploratory behaviour.Principal conclusionThe results obtained in the present study point to the importance of toxicity assessments in evaluating the efficiency of Cu NPs for the safe implementation in different applications. |
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| Keywords: | Copper nanoparticles Oxidative stress Acetylcholinesterase Wistar rats |
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