Inhibition of α-Glycosidase by Lippia dulcis Trevir. (Verbenaceae) Preparations,Quantification of Verbascoside,and Study of Its Molecular Docking |
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| Authors: | Natalia Ritter Ruas Aline Carvalho Pereira Luciana Lopes Silva Pereira Carolina Mesquita Germano Elaine Fontes Ferreira da Cunha Alexandre Alves de Carvalho Osmar Alves Lameira José Eduardo Brasil Pereira Pinto Suzan Kelly Vilela Bertolucci |
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| Institution: | 1. Department of Agriculture, Federal University of Lavras, Lavras, Brazil;2. PO Box 3037, Lavras, 37200-900 Minas Gerais, Brazil;3. Department of Medicine, Federal University of Lavras, Lavras, 37200-900 Minas Gerais, Brazil;4. Department of Chemistry, Federal University of Lavras, Lavras, 37200-900 Minas Gerais, Brazil;5. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Eastern Amazon Agroforestry Research Center, Belém, Brazil |
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| Abstract: | This study aimed to quantify verbascoside (VEB), perform molecular docking studies of VEB with the α-glucosidase (GL) of Bacillus stearothermophilus, and evaluate the inhibition of the enzyme by L. dulcis preparations. The substrate concentration and presence of reduced glutathione were evaluated for their effect on the in vitro inhibition of the GL enzyme. Assays were also performed in the presence and absence of simulated gastric fluid. The antidiabetic fractions 2 and 3 were the most inhibited GL, but their activity were significantly decreased in the presence of gastric fluid. Chromatographic analyses confirmed the predominant presence of VEB in the samples. The samples had VEB concentrations between 49.9 and 243.5 mg/g. Simulation of the molecular docking of VEB were consistent with its GL-inhibitory activity. It can conclude that the crude ethanol extract and fractions show inhibitory activity against the GL enzyme. |
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| Keywords: | medicinal plant ‘capim doce’ hypoglycemic agent glucosidase inhibitors phenylethanoid glycoside |
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