Identification of silicateins in freshwater sponge Lubomirskia baicalensis |
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| Authors: | O. V. Kaluzhnaya A. S. Belikova E. P. Podolskaya A. G. Krasko W. E. G. Müller S. I. Belikov |
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| Affiliation: | 1.Limnological Institute, Siberian Division,Russian Academy of Sciences,Irkutsk,Russia;2.Institute of Analytical Instrumentation,Russian Academy of Sciences,St. Petersburg,Russia;3.Institut für Psysiologische Chemie,Iohann Gutenberg Universit?t,Mainz,Germany |
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| Abstract: | Siliceous spicules of Baikal freshwater sponge Lubomirskia baicalensis contain several proteins, including silicateins. Analysis of a L. baicalensis cDNA library revealed four different mRNAs coding for proteins related to marine sponge silicatein α (α1, α2, α3, and α4). The intron-exon structure was determined forthe genomic α1 silicatein gene. The gene is 1988 bp from the initiation to the termination codon and consists of six intron (total size 1007 bp) and seven exons (total size 981 bp). Mass spectrometry of a tryptic digest of spicule proteins revealed peptides of two silicateins α. |
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| Keywords: | sponge Lubomirskia baicalensis biogenic silica silicatein intron-exon structure mass spectrometry tryptic digest |
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