不同人类免疫缺陷病毒感染途径群体中戊型肝炎病毒的流行情况

本研究旨在了解不同人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染途径群体中戊型肝炎病毒(hepatitis E virus,HEV)抗体情况,探讨HEV疫苗接种的必要性。采集HIV感染者的血清或血浆,利用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测HEV IgG抗体、IgM抗体及抗原,荧光定量聚合酶链反应(polymerase chain reaction,PCR)检测HEV核酸,Roche高纯化HIV-1核酸定量检测试剂盒(PCR荧光法)检测HIV感染者的HIV载量。比较分析不同HIV感染途径群体中HEV流行率的差别。结果显示,HIV感染者中HEV IgG抗体的阳性率为37.4%,静脉吸毒、成分献血和传播途径不明HIV感染群体的HEV IgG抗体阳性率分别为49.3%、39.5%和30.4%。HEV核酸荧光PCR检测结果均为阴性。3种HIV感染群体之间HEV IgG抗体阳性率差异无统计学意义(χ~2=2.978,P0.05)。HEV IgG阳性与阴性感染者之间HIV载量差异无统计学意义(P0.05)。结果提示,为保护HIV感染者免受HEV感染,应考虑接种HEV疫苗。

A serological study of hepatitis E virus infection in different human immunodeficiency virus-infected groups

In order to investigate the epidemic of hepatitis E virus (HEV) in human immunodeficiency virus (HIV )‐infected patients ,235 serum samples collected from HIV‐infected patients were subjected to molecular detections for HEV infection ,these including enzyme‐linked immunosorbent assay (ELISA) for HEV IgG and IgM and antigen , and polymerase chain reaction (PCR ) method for HEV RNA . Roche COBAS? AmpliPrep/COBAS? TaqMan HIV‐1 Test was applied for HIV levels . No HEV RNA‐positive results were found in this study .The positive rate of HEV IgG antibody in intravenous drug users ,blood component donors and unexplained HIV infection population were 49 .3% , 39 .5% and 30 .4% , respectively ,and the collective positive rate in HIV‐infected patients was 37 .4% .There was no statistically significant difference (χ2 = 2 .978 , P> 0 .05 ) in HIV viral load among three HIV‐infected groups , and between HEV IgG‐positive and‐negative populations (P>0 .05) .The results suggest that more studies are required to elucidate the role of HEV infection in HIV patients , and to establish effective preventive measures ,including vaccine inoculation for HIV‐infected patients .