Applications of thin layer chromatography,high performance liquid chromatography and mass spectrometry in the fermentation and isolation of the antibiotic nybomycin |
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| Authors: | Joseph A Pope Jr Richard A Nelson Carl P Schaffner Robert T Rosen Ramesh C Pandey |
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| Institution: | (1) Waksman Institute of Microbiology, Rutgers-The State University of New Jersey, Piscataway, NJ, U.S.A.;(2) The Center for Advanced Food Technology, Rutgers-The State University of New Jersey, New Brunswick, NJ, U.S.A.;(3) Xechem, Inc., A. Subsidiary of LyphoMed, Inc., Melrose Park, IL, U.S.A.;(4) Xechem, Inc., P.O. Box 459, 60048-9998 Libertyville, IL, U.S.A.;(5) Present address: Amgen, Inc., 1900 Oak Terrace Lane, 91320-1731 Thousand Oaks, California, U.S.A.;(6) Present address: The NutraSweet Company, 601 East Kensington Road, 60056-1363 Mt. Prospect, Illinois, U.S.A. |
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| Abstract: | Summary Thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and mass spectrometry (MS) methods have been developed for the analysis of the antibiotic nybomycin, its derivatives deoxynybomycin and nybomycin acetate, during the fermentation and isolation of nybomycin. Using a quantitative HPLC based assay, the time course of nybomycin production (nybomycin titers) in 1000 liter fermentations was determined. Desorption chemical ionization mass spectrometry (DCI/MS) of standard nybomycin samples, fermentation broth samples and purified fractions suggested the co-production of deoxynybomycin which was not reported previously from this organism. TLC and HPLC were used to confirm the presence of deoxynybomycin in the crude extracts of fermentation broths. |
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| Keywords: | Nybomycin Deoxynybomycin Nybomycin acetate Thin layer chromatography High performance liquid chromatography Mass spectrometry Fermentation |
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