| 枯草芽孢杆菌无标记遗传操作技术研究进展 |
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| 引用本文： | 朱旭军,李一凡,李杨,付晶,王智文,陈涛.枯草芽孢杆菌无标记遗传操作技术研究进展[J].微生物学通报,2015,42(12):2440-2447. |
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| 作者姓名： | 朱旭军 李一凡 李杨 付晶 王智文 陈涛 |
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| 作者单位： | 1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072,1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072,1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072,1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072,1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072,1．天津大学 化工学院 生物工程系 天津 300072;2．天津大学 教育部系统生物工程重点实验室 天津 300072 |
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| 基金项目： | 国家973计划项目(No. 2011CBA00804，2012CB725203)；国家自然科学基金项目(No. 21176182，21206112) |
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| 摘 要： | 枯草芽孢杆菌是革兰氏阳性菌的模式生物，长期以来在代谢工程和工业微生物领域扮演重要角色。枯草芽孢杆菌无标记遗传操作技术对后基因组时代的基因功能研究和菌株生理特性的改造起着关键作用。综述枯草芽孢杆菌无标记遗传操作所使用的负筛选标记基因，总结目前主流的无标记遗传操作的策略，并提出枯草芽孢杆菌无标记遗传操作技术面临的主要问题和发展方向。
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| 关 键 词： | 枯草芽孢杆菌，无标记遗传操作，负筛选标记，同源重组，位点特异性重组 |
Marker-free genetic manipulation technologies in Bacillus subtilis |
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| ZHU Xu-Jun,LI Yi-Fan,LI Yang,FU Jing,WANG Zhi-Wen and CHEN Tao.Marker-free genetic manipulation technologies in Bacillus subtilis[J].Microbiology,2015,42(12):2440-2447. |
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| Authors: | ZHU Xu-Jun LI Yi-Fan LI Yang FU Jing WANG Zhi-Wen and CHEN Tao |
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| Institution: | 1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China,1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China,1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China,1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China,1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China and 1. Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University,Tianjin 300072, China;2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, China |
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| Abstract: | Bacillus subtilis has been a model organism for gram-positive bacteria and has played important roles in metabolic engineering and industrial microbiology. Genetic manipulation technologies in Bacillus subtilis are critical for investigation of gene functions and modulation of bacteria physiologies in post-genomic era. In this review, we firstly summarize commonly used counter-selection markers for marker-free genetic manipulation. Then, we describe and compare different marker-free genetic manipulation strategies. Finally, we discuss challenges and future trends for developing more efficient tools for genetic manipulation of Bacillus subtilis. |
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| Keywords: | Bacillus subtilis Marker-free genetic manipulation Counter-selection marker Homologous recombination Site-specific recombination |
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