Towards the analysis of protein species: an overview about strategies and methods |
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Authors: | Peter R Jungblut Hartmut Schlüter |
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Institution: | 1.Core Facility Protein Analysis,Max-Planck-Institute for Infection Biology,Berlin,Germany;2.Institute of Clinical Chemistry - Mass Spectrometric Proteomics,University Medicine Hamburg-Eppendorf,Hamburg,Germany |
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Abstract: | The deciphering of the relationship between function and exact chemical composition of a defined protein species in the context
of the proteome is one of the major challenges in proteomics and molecular cell physiology. In the Special Issue of Amino
Acids about the analysis of protein species current approaches are reviewed and new methods described focusing on the investigation
of protein species. On the basis of the articles in this Special Issue it can be summarized that first important and promising
steps towards the comprehensive analysis of protein species have been done. It is already possible to obtain full (100%) sequence
coverage of proteins by mass spectrometry, if the amount of proteins available for their analysis allows their proteolytic
degradation by more than one protease and the subsequent mass spectrometric analysis of the resulting peptides. Employing
affinity chromatography helps to analyse proteins with defined post-translational modifications thus opening a targeted view
on e.g. the phosphoproteome. In the future the aim to identify the exact chemical composition including not one but every
posttranslational modification and complete sequence coverage on the protein species level should be achievable with further
progress in sample preparation techniques, especially concerning separation techniques on the protein level, mass spectrometry
and algorithms for mass spectrometric data processing. For determining the function of defined protein species a closer cooperation
between cell biologists and proteomics experts is desirable. |
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