| 单克隆抗体ELISA测定UVB诱导的DNA损伤(英文) |
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| 引用本文： | 李韶山,王艳,宾金华,王小菁,刘颂豪.单克隆抗体ELISA测定UVB诱导的DNA损伤(英文)[J].激光生物学报,1999(1). |
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| 作者姓名： | 李韶山 王艳 宾金华 王小菁 刘颂豪 |
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| 作者单位： | 华南师范大学激光生命科学研究所生物系，暨南大学水生生物研究所 |
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| 摘 要： | 本文采用单克隆抗体酶联免疫吸附分析法测定了ＵＶＢ诱导ＤＮＡ产生的ＣＰＤ和６４ＰＰ。经０．５ｍＷ／ｃｍ２ＵＶＢ处理１５ｍｉｎ的小牛胸腺和鲱鱼精ＤＮＡ，ＣＰＤ和６４ＰＰ含量显著增加，而未经ＵＶＢ处理的对照ＤＮＡ则没有二聚体形成。
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| 关 键 词： | UVB辐射 DNA损伤 环丁烷嘧啶二聚体（CPD） 6.4光产物（6-4PP） 酶联免疫吸附分析法（ELISA） |
Detection of DNA Damage Induced by UVB Radiation with Monoclonal Antibody ELISA |
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| LI Shaoshan,WANG Yan,BIN Jinhua,WANG Xiaojing and LIU Songhao.Detection of DNA Damage Induced by UVB Radiation with Monoclonal Antibody ELISA[J].ACTA Laser Biology Sinica,1999(1). |
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| Authors: | LI Shaoshan WANG Yan BIN Jinhua WANG Xiaojing and LIU Songhao |
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| Institution: | LI Shaoshan 1 WANG Yan 2 BIN Jinhua 1 WANG Xiaojing 1and LIU Songhao 1 |
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| Abstract: | By using monoclonal antibody specific for UV induced DNA damage, we have succeeded in measuring CPD and 64PP in UVB irradiated DNA. The results indicated that CPDs and 64PPs in the DNA were significantly increased by UVB radiation(0.5mW/cm 2 for 15min) as compared with that without UVB treatment. |
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| Keywords: | CPD DNA damage ELISA 64PP UVB radiation |
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