| 产唾液酸酶微生物的筛选及产酶条件的优化 |
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| 引用本文： | 曹丹,王学东,张鲁嘉,张建国,魏东芝.产唾液酸酶微生物的筛选及产酶条件的优化[J].生物加工过程,2009,7(4):40-45. |
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| 作者姓名： | 曹丹 王学东 张鲁嘉 张建国 魏东芝 |
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| 作者单位： | 华东理工大学,生物反应器工程国家重点实验室,上海,200237 |
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| 基金项目： | 国家高技术研究发展计划(863计划)重点资助项目 |
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| 摘 要： | 以无单唾液酸四己糖神经节苷脂（GM1）的神经节苷脂为唯一C源,从土壤中筛选出1株能以多唾液酸神经节苷脂为底物,转化生成GM1的产唾液酸酶微生物,经鉴定为溶黄嘌呤厄菌（Oerskovia xanthineolytica）。利用单因素法和响应面分析法对溶黄嘌呤厄菌产生唾液酸酶的条件进行优化,酶活提高了4.89倍。利用优化后培养条件,以神经节苷脂混合体为底物,经过微生物生物转化后,单唾液酸神经节苷脂GM1的含量从10%提高到83.7%。
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| 关 键 词： | 唾液酸酶 生物转化 单唾液酸四己糖神经节苷脂 溶黄嘌呤厄菌 |
Screening of sialidase-producing microorganism and optimization of sialidase production |
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| CAO Dan,WANG Xue-dong,ZHANG Lu-jia,ZHANG Jian-guo,WEI Dong-zhi.Screening of sialidase-producing microorganism and optimization of sialidase production[J].Chinese Journal of Bioprocess Engineering,2009,7(4):40-45. |
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| Authors: | CAO Dan WANG Xue-dong ZHANG Lu-jia ZHANG Jian-guo WEI Dong-zhi |
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| Institution: | (State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China) |
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| Abstract: | Oerskovia xanthineolytica-the strain which used to convert polysialoganglioside into monosialo-tetrahexosyl ganglioside(GM1) was efficiently screened from soil through high flux screening of sialidase-producing bacterium with free GM1 gangliosides as the sole source carbon.The concentration for maximum enzyme activity was optimized by single factor response surface analysis.The strain was cultured in the optimized medium containing crude gangliosides(10% w/v) at 37℃ for 12 h.Most of the polysialo-gangliosides are converted into GMl.The relative content of GM1 increased from 10% to 83.7% in the crude gangliosides. |
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| Keywords: | sialidase biotransformation monosialotetrahexosyl gangliosides Oerskovia xanthineolytica |
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