Effects of short-term treatment with calcium on the parathyroid gland of the rat,under particular consideration of the alteration of storage granules |
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Authors: | Takao Setoguti Yasuhisa Inoue Masasi Shin |
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Institution: | (1) Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan;(2) Department of Anatomy, Nagasaki University School of Medicine, 852 Nagasaki, Japan |
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Abstract: | Summary Short-term effects of CaCl2-treatment on parathyroid cells of the rat, especially on their storage granules, were studied at the ultrastructural level. After an injection of 4% CaCl2, serum calcium levels (SCL) rapidly increased from 9.1 mg/dl (controls) to a maximum of 14.9 mg/dl at 20 min. At 5 min after the injection, the number of type-I storage granules (large core) NSG-I] and that of type-II storage granules (small core) NSG-II] remained unchanged, in spite of elevated SCL (12.4 mg/dl). As soon as SCL rose to 13.2 mg/dl at 7.5 min, NSG-I gradually decreased to a minimum at 30 min; in contrast, NSG-II gradually increased to a maximum at 30 min. Vacuolar bodies also increased together with the augmentation of type-II storage granules. The average diameter of the core of the storage granules decreased significantly after the injection. Protein A-gold method for immunocytochemistry showed that the cores of these granules contain parathormone. Acid-phosphatase activity was occasionally found in storage granules of both types, especially in those of type II. It is concluded (i) that type-I storage granules may be transformed into vacuolar bodies via type-II granules as a result of hydrolysis, and (ii) that these processes may be accelerated during hypercalcemia. |
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Keywords: | Parathyroid Storage granule Hypercalcemia Immunocytochemistry Acid phosphatase Rat |
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