Competitive inhibition of T3 binding to alpha 1 and beta 1 thyroid hormone receptors by fatty acids. |
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Authors: | F R van der Klis E D Schmidt H C van Beeren W M Wiersinga |
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Institution: | Division of Endocrinology, Academic Medical Centre, Amsterdam, The Netherlands. |
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Abstract: | This study was undertaken to investigate whether fatty acids inhibit the binding of T3 to the alpha 1 and beta 1 form of the thyroid hormone receptor. Fatty acids inhibited the binding of T3 to both receptor proteins isolated from a bacterial expression system. The effectiveness of inhibition depends on the chain length and degree of saturation of the fatty acids. The inhibition of T3 binding to the alpha 1 and beta 1 receptor by oleic acid is competitive in nature; the Ki value was 5.4 10(-6) M for the c-erbA alpha 1 protein and 3.3 10(-6) M for the c-erb beta 1 protein. The findings indicate a direct interaction of fatty acids with T3 receptor proteins. |
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