The separation of major components of the casein of bovine milk by electrophoresis in a density gradient |
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Authors: | W Manson |
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Institution: | The Hannah Dairy Research Institute, Ayr |
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Abstract: | 1. Zonal electrophoresis in a column stabilized by a density gradient has been applied to the small-scale fractionation of the proteins of the casein complex of cow's milk. 2. The αs- and β-fractions from the milk of individual Ayrshire cows have been shown to behave as single homogeneous proteins on electrophoresis at two pH values in starch gels. 3. The αs-fraction has been found to be indistinguishable from αs-casein prepared by Ca2+ fractionation of the same milk samples. 4. On the evidence of their electrophoretic behaviour in starch gels and their elementary analyses, α1- and αs-casein are concluded to be substantially the same protein. |
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