|
|||||
|
|
Phosphatidylinositol-Specific Phospholipase C Production from Bacillus thuringiensis Serovar. kurstaki using Potato-Based Media |
|
| Authors: | T Palvannan R Boopathy |
| Institution: | (1) Department of Biotechnology, Kumaraguru College of Technology, Coimbatore, 641 006, Tamil Nadu, India;(2) Department of Biotechnology, Bharathiar University, Coimbatore, 641 046, Tamil Nadu, India |
| Abstract: | Summary Few studies on screening of the enzyme phosphatidylinositol-specific phospholipase C (PI-PLC) have been reported. Here we present a simple potato-based medium to elaborate large quantities of extracellular PI-PLC from Bacillus thuringiensis serovar. kurstaki. The enzyme activity (1.75 U/ml) was found to be maximum in the potato-extract medium supplemented with sucrose and mineral salts with a specific activity of 3.88 U/mg protein. |
| Keywords: | Acetylcholinesterases Bacillus thuringiensis phosphatidylinositol-specific phospholipases C screening triacyl-glycerol |
| 本文献已被 SpringerLink 等数据库收录！ | |