用ANS研究脱落酸提高植物线粒体膜的流动性

用荧光标记法，以ＡＮＳ作荧光探剂研究了脱落酸（ＡＢＡ）对植被线粒体脱流动性的影响。（＋）ＡＢＡ浓度效应实验结果和不同ＰＨ及不同温度条件下的实验结果均表明（＋）ＡＢＡ能降低植物线粒体膜的荧光强度，即（＋）ＡＢＡ能提高线粒体原流动性，且此种效应具有浓度饱和性，并导致线粒体异柠檬酸脱氢酶（ＩＣＨ）活性升高。ＡＢＡ的类似物，ＲＣＡ．７Ａ和ＲＣＡ．７ｂ，也具有提高ＩＣＤＨ活性的效应，抗ＡＢＡ结合蛋白的抗体（

RESEARCH ON INCREASE OF MEMBRANE FLUIDTY OF PLANTMITOCHONDRIA BY ABSCISIC ACID WITH ANS

The effect of abscisic acid (ABA) on the membrane fluidity of maize mitochondria was studied by the method of fluorescence labeling of 8-anilino-1-naphthalene-sulfonic acid (ANS). The results from the experiments of ( )ABA concentrations, pH and temperature showed that ( )ABA could all decrease the fluorescence intensity, namely, ( )ABA could enhance the membrane fluidity of ANS incubating with plant mitochondria. The saturation phenomenon was appeared in the effect of ( )ABA. Which resulted in the increase of isocitrate dehydrogenase (ICDH) activity. The analogs of ABA, RCA.7a and RCA.7b, could also increase the ICDH activity. However, polyclonal antibodies against ABA-binding protein (anti-ABBP PAbs) could inhibit such effect of ( )ABA. All of the above results suggested the existence the ABA receptor site in the mitochondria membrane.