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Development of the Particle Inflow Gun |
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| Authors: | Philippe Vain Noel Keen Jesus Murillo Carl Rathus Cheri Nemes John J Finer |
| Institution: | (1) Department of Agronomy, The Ohio Agricultural Research and Development Center, The Ohio State University, 44691 Wooster, OH, USA;(2) Department of Plant Pathology, University of California at Riverside, 92521-0122 Riverside, CA, USA;(3) Present address: CSIRO Cunningham Lab, 306 Carmody Rd., 4067 St Lucia Qld, Australia |
| Abstract: | A simple and inexpensive particle acceleration apparatus was designed for direct delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles directly in a helium steam. High levels of transient expression of theβ-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of maize and soybean, and leaf tissue of cowpea. Stable transformation of soybean and maize has also been obtained using this bombardment apparatus. |
| Keywords: | embryogenic suspension culture Glycine max particle bombardment stable transformation transient expression Zea mays |
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