Characterization of two novel pentose-fermenting and GABA-producing species: Levilactobacillus tujiorum sp. nov. and Secundilactobacillus angelensis sp. nov. Isolated from a solid-state fermented zha-chili |
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| Institution: | 1. Hubei Provincial Engineering and Technology Research Center for Food Ingredients, Hubei University of Arts and Science, Xiangyang, Hubei, PR China;2. Xiangyang Lactic Acid Bacteria Biotechnology and Engineering Key Laboratory, Hubei University of Arts and Science, Xiangyang, Hubei, PR China;3. Hubei Key Laboratory of Yeast Function, Yichang, Hubei, PR China;1. Federal University of Technology, Biochemistry Department, Enzyme and Food Biotechnology Unit, P.M.B. 704, Akure, Nigeria;2. Biological Science Department, Biochemistry Unit, Wesley University Ondo, Nigeria;1. Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia;2. Department of Biotechnology, Section of Environmental Biotechnology, TU Delft, The Netherlands;3. Institute of Polar Research, ISP-CNR, Messina, Italy;4. IAMC-CNR, Messina, Italy;5. NIOZ Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and Biogeochemistry, and Utrecht University, Utrecht, The Netherlands;1. School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, China;2. Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, Foshan University, Foshan 528225, China;3. School of Food Science and Engineering, Guangdong Ocean University, Yangjiang 529500, China;4. School of Science, RMIT University, GPO Box 2474, Melbourne, VIC 3001, Australia;5. Overseas Expertise Introduction Center for Discipline Innovation of Food Nutrition and Human Health (111 Center), Guangzhou 510640, China;1. Department of Pharmacology, MGV''s Pharmacy College, Panchavati, Nasik, 422003, Maharashtra, India;2. S K Biobiz Pvt. Ltd. Hall I-2, Sancheti Warehousing Complex, 10th Mile, Mumbai Agra Road, Jaulke, Tal. Dindori, Nasik, 422206, Maharashtra, India;1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;2. School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, People’s Republic of China;1. College of Life Sciences, Linyi University, Linyi 276000, China;2. College of Life Sciences, Yantai University, Yantai 264005, China;3. Weiyizhai Spice Food Co., Ltd, Linyi 276000, China |
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| Abstract: | Lactobacilli are dominant in zha-chili. This study provides a taxonomic characterization of five bacterial strains isolated from zha-chili in China. The cells were Gram-positive, facultative anaerobic, non-spore-forming, flagella-free, catalase-negative, heterofermentative, pentose-fermenting, and gamma-aminobutyric acid (GABA)-producing rods. For HBUAS51241T, HBUAS51329, and HBUAS51416, C16:0, C18:1 ω9c and C19:0 iso were the predominant cellular fatty acids; diphosphatidylglycerol (DPG), phosphatidylglycerol (DP), glycolipids (GL), and glycolipids (AL) were the major phospholipids. While for HBUAS51383T and HBUAS58055, C16:0, C18:1 ω9c, C19:0 cyclo ω8c were the predominant cellular fatty acids; DPG, DP, GL, and AL were the major phospholipids. Strains HBUAS51241T, HBUAS51329, and HBUAS51416 showed 98.1–99.1% 16S rRNA gene sequence similarity, 80.2–81.4% ANI, 87.7–90.0% AAI, and 23.8–32.8% digital DDH to their closest related type strains Levilactobacillus hammesii DSM 16381T, Levilactobacillus parabrevis ATCC 53295T, and Levilactobacillus fuyuanensis 244-4T. Strains HBUAS51383T and HBUAS58055 showed 98.7–99.5% 16S rRNA gene sequence similarity, 75.4–81.4% ANI, 75.5–89.1% AAI, and 19.7–24.0% digital DDH to their closest related type strains Secundilactobacillus silagincola IWT5T, Secundilactobacillus silagei JCM 19001T, Secundilactobacillus pentosiphilus IWT25T, Secundilactobacillus mixtipabuli IWT30T, Secundilactobacillus odoratitofui DSM 19909T, and Secundilactobacillus similis DSM 23365T. The central carbon metabolism pathways for the five strains were summarizeded. Based on the phenotypic, chemotaxonomic, and genomic data, we propose two novel species Levilactobacillus tujiorum sp. nov. whose type strain is HBUAS51241T (=GDMCC 1.3022T = JCM 35241T), and Secundilactobacillus angelensis sp. nov. whose type strain is HBUAS51383T (=GDMCC 1.3021T = JCM 35209T). |
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| Keywords: | Zha-chili Taxonomy Phylogenomy Central carbon metabolism pathways LAB"} {"#name":"keyword" "$":{"id":"k0040"} "$$":[{"#name":"text" "_":"Lactic acid bacteria ANI"} {"#name":"keyword" "$":{"id":"k0050"} "$$":[{"#name":"text" "_":"Average nucleotide identity HGT"} {"#name":"keyword" "$":{"id":"k0060"} "$$":[{"#name":"text" "_":"Horizontal gene transfer GI"} {"#name":"keyword" "$":{"id":"k0070"} "$$":[{"#name":"text" "_":"Genomic island dDDH"} {"#name":"keyword" "$":{"id":"k0080"} "$$":[{"#name":"text" "_":"digital DNA–DNA hybridization AAI"} {"#name":"keyword" "$":{"id":"k0090"} "$$":[{"#name":"text" "_":"average amino acid identity GABA"} {"#name":"keyword" "$":{"id":"k0100"} "$$":[{"#name":"text" "_":"gamma-aminobutyric acid mMRS"} {"#name":"keyword" "$":{"id":"k0110"} "$$":[{"#name":"text" "_":"modified de Man Rogosa and Sharpe NJ"} {"#name":"keyword" "$":{"id":"k0120"} "$$":[{"#name":"text" "_":"Neighbor-joining ME"} {"#name":"keyword" "$":{"id":"k0130"} "$$":[{"#name":"text" "_":"Minimum evolution ML"} {"#name":"keyword" "$":{"id":"k0140"} "$$":[{"#name":"text" "_":"maximum-likelihood |
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