Comparison of <Emphasis Type="Italic">Chloroflexus aurantiacus</Emphasis> strain J-10-fl proteomes of cells grown chemoheterotrophically and photoheterotrophically |
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Authors: | Li Cao Donald A Bryant Athena A Schepmoes Kajetan Vogl Richard D Smith Mary S Lipton Stephen J Callister |
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Institution: | (1) Biological Separations and Mass Spectrometry, Pacific Northwest National Laboratory, Richland, WA 99352, USA;(2) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA; |
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Abstract: | Chloroflexus aurantiacus J-10-fl is a thermophilic green bacterium, a filamentous anoxygenic phototroph, and the model organism of the phylum Chloroflexi.
We applied high-throughput, liquid chromatography–mass spectrometry in a global quantitative proteomics investigation of C. aurantiacus cells grown under oxic (chemoorganoheterotrophically) and anoxic (photoorganoheterotrophically) redox states. Our global
analysis identified 13,524 high-confidence peptides that matched to 1,286 annotated proteins, 242 of which were either uniquely
identified or significantly increased in abundance under photoheterotrophic culture condition. Fifty-four of the 242 proteins
are previously characterized photosynthesis-related proteins, including chlorosome proteins, proteins involved in the bacteriochlorophyll
biosynthesis, 3-hydroxypropionate (3-OHP) CO2 fixation pathway, and components of electron transport chains. The remaining 188 proteins have not previously been reported.
Of these, five proteins were found to be encoded by genes from a novel operon and observed only in photoheterotrophically
grown cells. These proteins candidates may prove useful in further deciphering the phototrophic physiology of C. aurantiacus and other filamentous anoxygenic phototrophs. |
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