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嗜酸氧化亚铁硫杆菌的高效培养及浸出黄铜矿初探
引用本文:康文亮,杨海麟,冯守帅,张玲,冷云伟,王武. 嗜酸氧化亚铁硫杆菌的高效培养及浸出黄铜矿初探[J]. 工业微生物, 2011, 41(4): 50-56. DOI: 10.3969/j.issn.1001-6678.2011.04.010
作者姓名:康文亮  杨海麟  冯守帅  张玲  冷云伟  王武
作者单位:1. 江南大学工业生物技术教育部重点实验室,江苏,无锡,214122
2. 中国矿业大学化工学院,江苏,徐州,221009
基金项目:江南大学青年基金,深圳市土木工程耐久性重点实验室开放课题
摘    要:采用向硫化矿培养基中补加FeSO4的方式以维持Fe2+ 浓度为4~8 g/L,可使嗜酸氧化亚铁硫杆菌菌浓在培养39 h时达到6.25×108 cells/mL,并在比生长速率几乎不降低的前提下提高了转化率和生产强度.然后对低氧化还原电位下低品位黄铜矿的浸出进行初步研究,结果表明经过30 d浸出,铜的浸出率可达28.5%...

关 键 词:嗜酸氧化亚铁硫杆菌  补料分批培养  硫化矿培养基  黄铜矿  生物浸出

Efficient culture of Acidithiobacillus ferrooxidans and preliminary study on chalcopyrite bioleaching
Kang Wen-liang,Yang Hai-lin,Feng Shou-shuai,Zhang Ling,Leng Yun-wei,Wang Wu. Efficient culture of Acidithiobacillus ferrooxidans and preliminary study on chalcopyrite bioleaching[J]. Industrial Microbiology, 2011, 41(4): 50-56. DOI: 10.3969/j.issn.1001-6678.2011.04.010
Authors:Kang Wen-liang  Yang Hai-lin  Feng Shou-shuai  Zhang Ling  Leng Yun-wei  Wang Wu
Affiliation:1. Key Laboratory of Industrial Bioteehnology of Ministry of Education, Jiangnan University, Wuxi 214122, China; 2. School of Chnical Engineering & Technology, China University of Mining and Technology, Xuzhou 221009, China)
Abstract:By feeding of ferrous sulfate into sulfide minerals medium to maintain the Fe2 + within the concentration range from 4 g/L to 8 g/L, the biomass of Acidithiobacillus ferroazidans could reach 6.25 ×10^8 cells/mL within 39 hours. At the same time the conversion rate and production intensity was improved without reducing the special growth rate. The preliminary study on low-grade chalcopyrite bioleaching at low redox potentials was carried out. The results showed that the leaching rate of copper could reach 28.5 percent within 30 days, and the Fe2+ , rather than sulfide minerals, was utilized as the bacteria growth energy in that leaching period.
Keywords:Acidithiobacillus ferrooxidans  fed-batch culture  sulfide minerals medium  chalcopyrite  bioleaehing
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