正交法优化嗜酸氧化亚铁硫杆菌冷冻干燥保护剂

利用正交实验方法,以甘油、海藻糖、蔗糖和牛血清蛋白为因素,对嗜酸氧化亚铁硫杆菌（Acididfiobacillus ferrooxidans,A．ferrooxidans）冷冻干燥保护剂的最优化配比进行了研究。直观分析、因素指标分析和方差分析的结果表明：由甘油、海藻糖、蔗糖和牛血清蛋白组成的冷冻干燥保护剂中,对存活率影响的主次顺序依次为：甘油〉海藻糖〉牛血清蛋白〉蔗糖。保护剂的最优化组合为甘油5％、海藻糖15％、蔗糖18％、牛血清蛋白10％。经过验证,该组合的保护剂可使冷冻干燥嗜酸氧化亚铁硫杆菌的存活率达到94％。

Freeze- drying Cryoprotectant Optimized by OrthogonalExperiment for Acidithiobacillus ferrooxidans

The optimized freeze-drying cryoprotectant of Acidithiobacillus ferrooxidans was studied by orthogonal experiment,in which glycerol,trehalose,sucrose and bovine serum albumin(BSA) were regarded as factors.The results of Direct-viewing analysis,the factor index analysis and variance analysis indicated that in freeze-drying cryoprotectant,which is composed of glycerol,trehalose,sucrose and BSA,the gradation of influencing survival rate is in turn as follows: glycerol > trehalose > sucrose > BSA.The optimized combination of freeze-drying cryoprotectant consists of 5%(w/v) glycerol,15%trehalose,18%sucrose and 10% BSA.,in which,the survival rate of Acidithiobacillus ferrooxidans could reach 94%.