Improved genetic transformation protocol for cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) |
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Authors: | Rubén Álvarez Ricardo Javier Ordás |
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Institution: | (1) Departamento de Biología de Organismos y Sistemas, Universidad de Oviedo, C/. Catedrático Rodrigo Uría s/n, Oviedo, 33011, Spain |
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Abstract: | An optimized protocol for Agrobacterium tumefaciens-mediated transformation of mature Quercus suber L. embryogenic masses is reported. In this work several variables were tested. Plant genotype, explant type and time elapsed
between the last subculture and inoculation, i.e. the explant preculture period, were found to be very important. Interaction between inoculum density and cocultivation period
influenced the transformation efficiency as well. A transformation efficiency (i.e. percentage of the inoculated explants
that yielded independent transgenic embryogenic lines) of up to 43% was obtained, greatly improving the previously described
method for plant transformation of adult-selected cork oak. It was also shown that this protocol could be applied to various
genotypes.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Agrobacterium Fagaceae Somatic embryogenesis Tree genetic transformation |
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